《植物生理学报》 2013, 49(6): 586-590

高盐和高温胁迫下外源脯氨酸对PSII颗粒的保护作用

邱念伟^1,*, 杨翠翠^1,*, 付文诚¹, 胡胜¹, 周峰^2,**

1曲阜师范大学生命科学学院, 山东曲阜273165; 2南京晓庄学院生物化工与环境工程学院, 南京211171

通信作者：邱念伟；E-mail: zfibcas@163.com

摘　要：

为研究脯氨酸在植物耐盐性和耐热性中的作用, 提取菠菜叶片的PSII颗粒, 进行高盐(100~800 mmol·L-1 NaCl)、高温(30和40 ℃)以及盐和热交叉胁迫处理。PSII最大光化学效率(Fv/Fm)的测定结果表明, 0 ℃下, 高盐胁迫处理对PSII颗粒伤害较小; 无盐胁迫下, 30 ℃处理对PSII伤害不明显, 40 ℃高温处理则显著降低PSII活性; 盐和热交叉胁迫加剧了对PSII的伤害。在PSII颗粒的保存液中加入脯氨酸(100~800 mmol·L-1)能显著提高PSII在高盐胁迫以及盐和热交叉胁迫下的活性, 但未能提高其在高温胁迫下的活性。这一结果为脯氨酸提高植物耐盐性及对盐和热交叉胁迫抗性方面的功能研究提供了直接证据。

关键词：脯氨酸; 光系统II; 高盐胁迫; 高温胁迫; PSII最大光化学效率

收稿：2013-03-18   修定：2013-04-24

资助：作物生物学国家重点实验室开放课题(2011KF07)和江苏省自然科学基金青年基金项目(BK2012073)。

Protective Effect of Exogenous Proline on PSII Particles under High Salinity and High Temperature Stresses

QIU Nian-Wei^1,*, YANG Cui-Cui^1,*, FU Wen-Cheng¹, HU Sheng¹, ZHOU Feng^2,**

¹College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China; ²School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China

Corresponding author: QIU Nian-Wei; E-mail: zfibcas@163.com

Abstract:

To explore the role of proline in salt tolerance and heat tolerance of plants, photosystem II (PSII) BBY particles prepared from spinach (Spinacia oleracea) leaves were treated with different concentrations of NaCl (100–800 mmol·L-1), different temperatures (30 and 40 ℃) and cross treatments of NaCl and high temper-ature. Then maximal efficiency of PSII photochemistry (Fv/Fm) was determined by plant efficiency analyser (PEA). The results showed that single salt-treatment caused slight damage to PSII at 0 ℃, and 30 ℃ treatment almost had no damage to PSII under 0 mmol·L-1 NaCl condition, but PSII activity (Fv/Fm) decreased markedly at 40 ℃. While under cross treatments of salt and high temperature, PSII activity decreased more significantly than under the single stress. Addition of 100–800 mmol·L-1 proline in store buffer could greatly increase the ac-tivity of PSII under salt stress and cross stress of salt and high temperature, but could not increase the activity of PSII under high temperature stress. These results above provide direct evidence that accumulation of proline can enhance salt tolerance and cross-tolerance to salt and high temperature in plants.

Key words: proline; photosystem II; high salinity stress; high temperature stress; maximal efficiency of PSII photochemistry